Artron Gene True Bar & CP4 EPSPS Combo Test Kit

Catalog Number: A07-23-413

INTENDED USE:

Artron GeneTrue PAT & CP4 EPSPS Combo Test Kit is intended to determine the presence of the transgenic PAT/bar protein and/or the CP4 EPSPS protein in genetically modified organisms.

DESCRIPTION:

Transgenic PAT/bar protein when expressed in plants confers plant resistance to herbicides; the test kit is able to detect the presence of the PAT/bar protein expressed in transgenic plants. Experienced researchers can determine the expression level based on the color revealed on the strip test line, allowing for a qualitative or semi-quantitative analysis relative to the degree to which the PAT/bar protein is expressed in a plant.
EPSPS or 5-enolpyruvylshikimate-3-phosphate synthase is an enzyme that catalyzes a reaction in plant and bacterial cells that is necessary for synthesizing some amino acids. The herbicide, Roundup (glyphosate), can bind to the EPSPS enzyme made in plants and block its ability to work. This causes the plant to run out of amino acids which halts growth and development. The plant eventually dies from starvation. Roundup is a non-selective systemic herbicide which means it injures and often kills most plants that come into contact with it.
CP4 EPSPS is the protein introduced by genetic manipulation that is expressed in glyphosate-tolerant soybeans, which are being developed to provide new weed-control options for farmers. Expression of this protein in plants imparts high levels of glyphosate tolerance. The safety of CP4 EPSPS was ascertained by evaluating both physical and functional characteristics. CP4 EPSPS degrades readily in simulated gastric and intestinal fluids, suggesting that this protein will be degraded in the mammalian digestive tract upon ingestion as a component of food or feed.

SAMPLE TYPE:

Time to Results:
2~5 min
Analytical Sensitivity:
1 ng/ml or 1 in 1000 kernels
Analytical Specificity:
No cross reactions were observed from all tested specimens
Interference:
No interference from common biological and chemical analytes
Diagnostic Sensitivity:
Diagnostic Specificity:
Accuracy:
Precision
The precision was determined by replicate assays of both positive and negative samples with devices from three different production lots. The resultant data indicated no appreciable between lot variation when testing both positive and negative samples across three different lots.
Storage condition:
Store at 2-30°C, avoid direct sunlight and freezing.
Shelf life:
12 months
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WARNINGS AND PRECAUTIONS
  • For professional in vitro diagnostic use only.
  • Do not reuse.
  • Do not use if the product is sealed barrier or its packaging is compromised.
  • Do not use it after the expiration date shown on the pouch.
  • Do not mix and interchange different specimens.
  • Wear protective clothing such as laboratory coats, disposable gloves, and eye protection while handling potentially infectious material and performing the assay.
  • Wash hands thoroughly after finishing the tests.
  • Do not eat, drink or smoke in the area where the specimens or kits are handled.
  • Clean up spills thoroughly with appropriate disinfectants.
  • Handle all specimens as if they contain infectious agents. Observe established precautions against microbiological hazards throughout testing procedures.
  • Dispose of all specimens and used devices in a proper container. The handling and disposal of hazardous materials should follow local, national, or regional regulations.
  • Keep out of children’s reach.
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