Artron’s one-step FSH Menopause Test is an antigen-capture immunochromatographic assay, that detects the presence of FSH in human urine samples.
Monoclonal antibodies specifically against FSH are 1) conjugated with colloidal gold and deposited on the conjugate pad and 2) immobilized on the test line on the nitrocellulose membrane.
When the urine sample is added the gold-antibody conjugate is rehydrated and the FSH if any in samples, interacts with the gold-conjugated antibodies. The antigen-antibody-gold complex will migrate towards the test window until the Test Zone (T) where they will be captured by immobilized antibodies, forming a visible pink line (Test band, indicate positive results). If FSH is absent in the sample, no pink line will appear in the Test Zone (T), indicating a negative result.