Artron One Step HBsAg test is an antigen-capture immunochromatographic assay, that detects the
presence of HBsAg in blood samples. Monoclonal antibodies specifically against HBsAg are 1)
conjugated with colloidal gold and deposited on the conjugate pad and 2) immobilized on the test
line on the nitrocellulose membrane. When the blood sample is added, it rehydrates the gold-
antibody conjugate and the HBsAg, if any in the sample, interacts with the gold-conjugated
antibodies. The antigen-antibody-gold complex will migrate toward the test window until the Test
Zone (T) where they are captured by immobilized antibodies, forming a visible pink line (Test band,
indicates positive results). If HBsAg is absent in the sample, no pink line will appear in the Test
Zone (T).
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