What are Sandwich Immunoassays?

Chemiluminescence immunoassays are a type of sandwich immunoassay that use two binding partners to capture and detect an analyte of interest. They are especially useful for measuring large molecules that have more than one antigenic determinant, such as antibodies or antigens.

What are Sandwich Immunoassays?

How do sandwich immunoassays work?

The sandwich method is a type of test that can measure large molecules that have two or more binding sites. The sandwich method has two subtypes: the double-antibody sandwich method and the double-antigen sandwich method. The difference lies in the type of binding partners that are used to form the sandwich complex.

Double-antibody sandwich method

In this method, the sample molecules are trapped between two antibodies that recognize different parts of the molecule. One antibody is attached to a solid surface and the other antibody is labeled with acridine, a chemical that can produce light.

Double-antigen sandwich method

In this method, the sample molecules are antibodies that are trapped between two antigens that recognize different parts of the antibody. One antigen is attached to a solid surface and the other antigen is labeled with acridine.

In both methods, a solution of hydrogen peroxide and sodium hydroxide is added to make the acridine release light. The amount of light is measured by an optical system and is related to the amount of sample molecule in the test.

What are Sandwich Immunoassays?

The applications of sandwich immunoassays

Sandwich immunoassays are a powerful tool for the analysis of large molecules that have multiple antigenic determinants. They are widely used in various fields and applications, such as clinical diagnostics, biomedical research, food safety, and environmental monitoring.

As a provider of immunoassay products, we want to introduce our compact Automatic Chemiluminescence Immunoassay Analyzer, iA1000. The machine can throughput up to 120 tests/hour and has automatic dilution with 1:2 – 1:40 within one operation.

Learn more about iA1000 CLIA here.

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