Artron One-Step Luteinizing Hormone (LH) Test is an antigen-capture immunochromatographic assay, which detects the presence of LH in human urine samples. Monoclonal antibodies specifically against LH are 1) conjugated with colloidal gold and deposited on the conjugate pad and 2) immobilized on the test line of the nitrocellulose membrane. When the urine sample is added the gold-antibody conjugate is rehydrated and the LH if any in samples, interacts with the gold-conjugated antibodies. The antigen-antibody-gold complex will migrate towards the test window until the Test Zone (T) where it will be captured by immobilized antibodies, forming a visible pink line (Test band), indicating a positive result of LH surge. If LH is absent in the sample, no pink line will appear in the Test Zone (T), indicating a negative result. To serve as an internal process control, a control line should always appear at Control Zone (C) after the test is completed. The absence of a pink control line in the Control Zone is an indication of an invalid result.